The cost reduction from Inquisitive Glimmer's first ability applies only to generic mana in the total cost of enchantment spells you cast. Similarly, the cost reduction from its second ability applies only to generic mana in the unlock costs of Rooms you control. (In other words, it's impossible for these abilities to combine to make anything cost less.)

To determine the total cost of a spell, start with the mana cost or alternative cost you're paying (such as a flashback cost), add any cost increases (such as kicker costs), then apply any cost reductions (such as that of this ability). The mana value of the spell is determined by only its mana cost, no matter what the total cost to cast that spell was.