When an effect suspects a creature, it becomes suspected. It gains menace and "This creature can't block" for as long as it's suspected. It stays suspected until it leaves the battlefield or another effect causes it to no longer be suspected.

If a creature is already suspected, suspecting it again won't have any effect.

If a suspected creature loses all abilities, it will lose menace and "This creature can't block", but it won't stop being suspected.

If Rune-Brand Juggler is suspected, you can sacrifice it to pay for its own last ability.

Being suspected isn't a copiable value. If a permanent becomes a copy of a suspected creature, it won't be suspected.

There's no limit to the number of creatures that can be suspected simultaneously. Suspecting a new creature doesn't cause other creatures to stop being suspected.